Biochem/physiol Actions

Primary TargetCalmodulin-Dependent Protein Kinase (CaM Kinase)-2

Target IC₅₀: 50 nM against cam Kinase-2

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

General description

The myristoylated form of CaMK IINtide (Cat. No. 208920). The peptide has been modified at the amino terminal lysine with the addition of three glycine residues and myristoylated to improve cell-permeability.

The myristoylated form of CaMK IINtide (Cat. No. 208920), a potent, specific inhibitor of Ca²⁺/Calmodulin kinase II (CaMK II) (IC₅₀ = 50 nM for total and Ca²⁺-independent CaMK II activity). The peptide sequence corresponds to the inhibitory domain of the CaMK II inhibitory protein, CaMK IIN. Exhibits inhibitory activity across converged species, including rat brain, goldfish brain, and Drosophila (IC₅₀ = 100-400 nM). Shown to completely inhibit the phosphorylation of GluR1 fusion protein at a concentration of 1 µM. Does not inhibit CaMK I, CaMK IV, CaMKK, PKA, or PKC. The peptide has been modified at the amino terminal lysine with the addition of three glycine residues and myristoylated to improve cell-permeability.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sodering, T.R., et al. 2001. J. Biol. Chem.276, 3719.Chang, B.H., et al. 1998. Proc. Natl. Acad. Sci. USA95, 10890.Pereda, A.E., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13272.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at-20°C.

Sequence

Myr-N-Gly-Gly-Gly-Lys-Arg-Pro-Pro-Lys-Leu-Gly-Gln-Ile-Gly-Arg-Ala-Lys-Arg-Val-Val-Ile-Glu-Asp-Asp-Arg-Ile-Asp-Asp-Val-Leu-Lys-OH

Warning

Toxicity: Standard Handling (A)